Πρόβλεψη της γλυκαιμικής απόκρισης: μελέτη in vitro σε μοντέλα συστήματα και σε τρόφιμα

Abstract

Diabetes mellitus is for several years object of interest and study of the scientific community because it’s increased prevalence. Fiber consumption causes decreased postprandial glucose and insulin responses. The present study suggests an in vitro methodology that simulates the normal conditions of digestion for carbohydrates and predicts the postprandial glucose concentration. The suggested methodology involves 4 stages (oral cavity, stomach, a transitional phase adjustment pH and intestinal digestion) in vitro digestion for food or mix foods at different pH, presence of enzymes and using dialysis membrane. The total time of in vitro digestion is 4 1/2 hours. The first step of this study was the addition a-cyclodextrin in increasing doses (0.01g, 0.025g and 0.05g α-cyclodextrin) in samples of white bread. The samples of white bread with 0.05g αcyclodextrin resulted in decreased absorption of glucose at 90 min (p=0.011) and at 120min (p=0.046). Subsequently, samples were selected for in vitro digestion from the International table of Glycemic Index and Glycemic Load Values. Stronger statistically significant correlation with the Glycemic Index in vivo was observed with the absorption of glucose at t=120min (Spearman’s rho=0,883). Moreover, the absorption of glucose at t=120min correlated with the values of glycemic load, giving very good results (Spearman’s rho=0,953).